Johnston-Ogston effects in AUC simulations of two model systems based on polystyrene beads that are polydisperse with respect to specific gravity.
Link to the latest draft: PS bead draft of February 27, 2016. (First published on February 17, 2012.)
This document applies material from An irreversible thermodynamic description of analytical ultracentrifugation (AUC) applied to a solution of the time- and gravitational-potential-space-dependent Lamm equation, to which a link is provided in an accompanying post (Irreversible thermodynamics of AUC and MCE). Simulation results include those obtained using a simple coupled-flow equation for AUC.
Selected data sets from this document are analysed in The apparent sedimentation coefficient, s*, and its distribution function, g(s*), within -∞ < s* < ∞, to which a link is provided in an accompanying post (g(s*) within -∞ < s* < ∞). The two model systems described in this document are used to create the four treatment groups of Distinguishing hypothetical systems of PS beads by g(s*) analysis of simulated AUC data to which noise has been added, and quantifying the statistical significance of any observed distinguishability, to which a link is also provided in an accompanying post (Effects of noise on the distinguishability of several simulated AUC systems of PS beads).
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